Abcam, ab269372, Human Fetuin-A ELISA Kit

Size: 1 x 96Tests
Human Fetuin-A ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Fetuin-A with a sensitivity of 116.24 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 116.24 pg/mL,
Range:203.13 - 13000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Fetuin-A ELISA Kit (ab269372) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fetuin-A protein in cell culture supernatant, edta plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fetuin-A with 116.24 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Fetuin A is a highly abundant protein secreted by the liver that is capable of binding a wide variety of ligands, similar to serum albumin. A member of the cystatin family, Fetuin A plays a vital role in preventing tissue calcification and is also involved in fatty acid transport, insulin regulation and anti-inflammatory responses. Fetuin A is encoded by a single gene on chromosome 3, contains two cystatin domains, and exists as a heterodimer in its posttranslational form. Fetuin A is more abundant than serum albumin in fetal serum.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target AHSG also known as fetuin-A or alpha-2-HS-glycoprotein is a multifunctional glycoprotein with an approximate mass of 64-68 kDa. It is mainly expressed in the liver and secreted into the bloodstream. AHSG exhibits a wide range of interactions with different cell types and biological molecules playing key roles in mineralization inhibition of arterial calcification and modulation of immune responses.
Biological function summary
AHSG protein serves multiple functions including acting as an inhibitor of insulin receptor autophosphorylation and serine-threonine kinase activities. AHSG is part of a complex dynamic process involving calcium metabolism and bone mineralization. It binds to transforming growth factor-beta (TGF-β) and other proteins modulating cellular signals and maintaining homeostasis in tissues. The regulation of calcium ion balance by AHSG can protect against ectopic calcification preventing potential damage.
Pathways
Calcium signaling and the insulin signaling pathways integrate AHSG functions. AHSG interacts with proteins like insulin receptor and those in the canonical Wnt signaling pathway influencing glucose metabolism and bone development. The balance of its interactions in these pathways helps maintain metabolic homeostasis and skeletal integrity reflecting its versatile roles within complex networks.
AHSG has connections to metabolic syndrome and calcific aortic valve disease. Abnormal levels of AHSG can disrupt metabolic functions and contribute to type 2 diabetes development by affecting insulin receptor activity. It also associates with calcification-related proteins in vascular tissues linking it to cardiovascular diseases. Understanding these connections is important for potential therapeutic targeting of AHSG in related pathologies.