Abcam, ab269492, Anti-T2A + P2A antibody [3H4] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal antibody. Carrier free. Suitable for WB, ICC/IF, Dot and reacts with Synthetic peptide samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3H4,
Isotype:IgG,
Carrier free:Yes,
Applications:WB, ICC/IF, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The T2A and P2A sequences commonly referred to as 2A peptides function as self-cleaving peptides that facilitate the expression of multiple proteins from a single gene construct. These peptides act as short sequences of amino acids that cause ribosomal skipping during translation resulting in discrete protein products. T2A and P2A are used as linker sequences and their use is often interchangeable in molecular biology techniques for polycistronic expression systems. The T2A peptide is derived from the Thosea asigna virus while P2A originates from the Porcine teschovirus-1. These sequences are small with a mass typically around 2 kDa and they are primarily expressed where polycistronic vectors are utilized such as in transgenic organisms or recombinant protein production systems.
Biological function summary
T2A and P2A peptides play a significant role in the facilitation of protein co-expression in a variety of organisms. They do not form part of any multi-protein complex but enable independent expression of protein components from within a single vector by cleaving themselves during protein translation. This ability is especially beneficial for engineering purposes where researchers require stable gene expression and production of multiple proteins simultaneously without the complication of internal ribosomal entry sites (IRES) or additional subunits.
Pathways
T2A and P2A peptides do not directly belong to classical biological pathways as they do not exist as conventional proteins. Instead they influence pathways facilitated by the proteins they help express. These peptides are employed in reverse engineering cellular pathways where simultaneous gene expression is desired such as the Wnt signaling pathway or apoptosis regulation. Both pathways involve key proteins that can be expressed using T2A and P2A sequences allowing researchers to construct and study artificial biological systems with enhanced control.
T2A and P2A peptides find significance in the study and therapeutic targeting of conditions like cancer and neurodegenerative diseases. In cancer research for instance these peptides enable the co-expression of oncogenes and tumor suppressors helping to elucidate their roles in tumorigenesis. Neurodegenerative disorders such as Parkinson's disease also benefit from their application as T2A and P2A sequences allow for the expression of multiple therapeutic proteins including growth factors like GDNF offering insights into potential combinatorial treatments. By facilitating multi-gene expression these peptides are integral to advancing understanding and therapeutic developments in complex diseases.