Abcam, ab270111, Alexa Fluor® 647 Anti-HAND2 antibody [EPR19451]

Size: 100µL
Rabbit Recombinant Monoclonal HAND2 antibody - conjugated to Alexa Fluor® 647. Suitable for ICC and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19451,
Isotype:IgG,
Conjugation:Alexa Fluor® 647,
Excitation/Emission:Ex: 650nm, Em: 665nm,
Carrier free:No,
Reacts with:Mouse,
Applications:ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HAND2 also known as Heart and Neural Crest Derivatives Expressed 2 is a transcription factor weighing approximately 22 kDa. This protein plays an important role in regulating the expression of genes necessary for specific cell differentiation processes. HAND2 is widely expressed in cardiac and neural tissues with a particular emphasis on the developing heart and nervous system. It belongs to the basic helix-loop-helix (bHLH) family of transcription factors which are known for their function in binding to E-box sequences in DNA and controlling gene expression.
Biological function summary
HAND2 influences cardiac morphogenesis and contributes to neural crest cell migration. It engages in complex interactions with other transcription factors to modulate the expression of genes involved in these processes. HAND2 operates as part of a transcriptional complex often forming heterodimers with other bHLH proteins like E47 and HEB which enhances its DNA-binding capability and specificity. This interaction is essential for the activation of downstream targets that control cellular differentiation and proliferation in cardiac and neural development.
Pathways
HAND2 is an important participant in both heart development and neural crest cell signaling. It integrates signals in the BMP (Bone Morphogenetic Protein) pathway and partners with proteins such as NKX2-5 and GATA4 which are critical for orchestrating cardiac gene expression. HAND2 also plays a significant role in the WNT signaling pathway contributing to the proper guidance and differentiation of neural crest derivatives.
HAND2 mutations or dysregulation have links to congenital heart defects and neural crest-related disorders. In congenital heart defects altered HAND2 function impacts the expression levels of NKX2-5 and GATA4 leading to improper heart formation. Additionally HAND2's involvement in neural crest cell development associates its dysregulation with diseases such as Hirschsprung's disease. Pathological changes in HAND2 activity potentially disrupt the normal neural crest migration patterns contributing to these developmental anomalies.