Abcam, ab270217, Human Glypican 1 ELISA Kit (GPC1)

Size: 1 x 96Tests
Human Glypican 1 ELISA Kit (GPC1) is a single-wash 90-min Simplestep used to quantify Human Glypican 1 (GPC1) with a sensitivity of 16.16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Urine, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 16.16 pg/mL,
Range:78.13 - 5000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Glypican 1 ELISA Kit (GPC1) (ab270217) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Glypican 1 (GPC1) protein in cell lysate, edta plasma, serum, urine, cell culture extracts, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Glypican 1 (GPC1) with 16.16 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human Glypican 1 (GPC1) is synthesized as a 558 amino acid (aa) precursor that contains a 23 aa signal sequence, a 507 aa mature segment, and a 28 aa C-terminal prosegment. The standard protein in this product is the mature form. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. GPC1 may play a role in the control of cell division and growth regulation. Human GPC1 shares 89%, 89%, and 87% sequence homology with mouse, rat, and bovine, respectively.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glypican 1 (GPC1) also known as glypican-1 is a heparan sulfate proteoglycan anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. This protein belongs to the glypican family and has an approximate mass of 60 to 70 kDa. GPC1 is found mainly on the surface of cells in the central nervous system as well as in muscle tissues and certain epithelial cells. Its structure facilitates interaction with cell surface receptors and extracellular matrix components playing an important role in cellular communication and signaling processes.
Biological function summary
GPC1 influences the regulation of growth factors and morphogens and it has a significant role in the cell division and growth processes. It is not recognized as part of any larger protein complex but interacts with diverse ligand molecules. Researchers identify GPC1 as an important facilitator of fibroblast growth factors (FGF) and sonic hedgehog (Shh) signaling. Its interaction with molecular partners helps to regulate developmental signals important for proper cellular function and organ development.
Pathways
The contributions of GPC1 are observed in the hedgehog signaling pathway and fibroblast growth factor signaling. GPC1's interaction within the hedgehog pathway is critical for organizing cellular growth and differentiation while its involvement in the FGF pathway supports angiogenesis and wound healing. GPC1 coordinates with other glypican family members and related proteins like FGFR1 to mediate these signaling processes effectively.
Researchers have linked GPC1 with cancer progression and neurodegenerative diseases. Abnormal GPC1 expression is often noted in pancreatic cancer influencing tumor growth and metastasis through its modulation of growth factor signaling. Moreover its role in neurodegenerative conditions such as Alzheimer's disease establishes connections with amyloid-beta peptides and tau proteins contributing to disease pathogenesis. These associations make GPC1 an intriguing target for therapeutic intervention and a focus for ongoing medical research.