Abcam, ab270309, Protein G Resin - Amintra

Size: 5mL / 25mL / 100mL
Protein G Resin - Amintra (ab270309) is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
Key facts
Form:LiquidSee storage information,
Storage buffer:pH: 6 - 8Constituents: 50% Agarose beads, 20% Ethanol

Product details:
Protein G Resin - Amintra (ab270309) is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to highly cross-linked 4% agarose. The binding capacity of Protein G Resin - Amintra is greater than 30mg human lgG/mL medium. With the high binding capacity and chemical stability, very high batch-to-batch reproducibility, which ensures excellent performance on isolation of immune complexes.
This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Protein G Resin. Expedeon product codes APG0005 (5 mL Medium), APG0025 (25 mL Medium) and APG0100 (100 mL Medium) are the same as the current sizes: 5 mL, 25 mL, and 100 mL, respectively.
Characterization of Resin:
Supporting matrix:
Highly crosslinked 4% agarose supplied as a 50% slurry
Ligand:
Recombinant Protein G
Bead size range:
45-165 μm
pH stability:
long term: pH 3 – 9 –short term: pH 2 – 10
Typical binding capacity:
>30 mg Human IgG /ml resin
Maximum pressure:
0.3MPa (3 bar)
Chemical stability:
High. The IgG binding capacity and recovery was maintained after storage for:
7 days at 37° in:
1 M acetic acid pH 2.0, 20 mM sodium phosphate, 1% SDS, pH 7.0
6 M Guandine-HCl, pH 7.0
70% ethanol
2 hours at room temperature in:
0.1 M HCl, pH 1.0
8 M urea, pH 10.5
0.1 M Glycine-NaOH, pH 11.0
Solubility in water:
Insoluble

Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze