Abcam, ab270325, Anti-LBP antibody [EPR22559-90] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal LBP antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22559-90,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse,
Applications:sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab270325 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse LBP. The recommended pair for sandwich ELISA is:
Capture:
ab270324
, Mouse LBP Capture Antibody (unconjugated)
Detector: ab270325, Mouse LBP Detector Antibody (unconjugated)
The reference range value is 50 - 3200 ng/ml.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lipopolysaccharide-binding protein (LBP) also known as LPS-binding protein is an acute-phase protein that plays a critical role in the immune response to Gram-negative bacterial infections. This protein weighing approximately 60kDa is primarily expressed in the liver and found in the serum. LBP binds to lipopolysaccharides (LPS) which are components of the outer membrane of Gram-negative bacteria facilitating the transfer of LPS to CD14 receptors on immune cells. This transfer enhances the recognition of bacterial endotoxins triggering an immune response.
Biological function summary
LBP serves a significant role in enhancing the sensitivity of the immune system to bacterial endotoxins. It does not form a permanent part of a larger complex but works closely with other molecules like CD14 and MD-2 an accessory protein of the TLR4 receptor to mediate the innate immune response. By efficiently binding LPS LBP aids in transporting these molecules to cell surface receptors accelerating the detection and subsequent response to infections.
Pathways
LBP is heavily involved in the TLR4 signaling pathway which is important for the detection and response to Gram-negative bacterial infections. Through this pathway LBP and associated proteins such as CD14 and MD-2 help activate TLR4 triggering downstream NF-κB signaling. This activation leads to the production of pro-inflammatory cytokines and an upsurge in antimicrobial activities fortifying the body's defenses against bacterial invasion.
Enhanced LBP levels have been associated with sepsis a severe systemic response to bacterial infections which can often lead to septic shock. Given its pivotal role in recognizing bacterial endotoxins LBP is important in identifying infections making it a potential prospect for therapeutic targeting. Additionally its interaction with CD14 means that alterations in LBP function or expression levels might contribute to immune dysregulation seen in inflammatory diseases such as Crohn's disease where immune responses to gut microbiota play a central role.