Abcam, ab270354, Human HRAS Antibody Pair - BSA and Azide free

Size: 10 x 96Tests
Human HRAS Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human HRAS.
Key facts
Reacts with:Human,
Assay type:ELISA set,
Range:125 - 8000 pg/mL,
Assay Platform:Reagents

Product details:
The Antibody Pair can be used to quantify Human HRAS. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (
ab267797
), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery
Bead-based assays
AlphaLISA
/AlphaScreen
DELFIA
immunoassays
and Single Molecule Counting (SMC™) immunoassays
Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody:
recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody:
recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration
: ~1 mg/ml
Storage buffer:
100% PBS
Liquid
Isotype:
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GTPase HRAS also known as H-RAS or HRAS1 is a small GTPase enzyme involved in intracellular signal transduction. It has a molecular mass of approximately 21 kDa. HRAS is widely expressed in various tissues and plays a role in regulating cell growth and differentiation. The HRAS protein functions as a molecular switch cycling between an active GTP-bound state and an inactive GDP-bound state. This activity is facilitated by the HRAS protein's intrinsic GTPase activity which hydrolyzes GTP to GDP.
Biological function summary
GTPase HRAS participates in the regulation of cell proliferation differentiation and survival serving as an essential component of several signal transduction complexes. HRAS interacts with various signaling molecules and pathways to mediate mitogenic signals. Through these interactions the HRAS protein modulates cellular responses to extracellular stimuli influencing processes like growth and division playing an important role in maintaining normal cellular functions.
Pathways
The involvement of GTPase HRAS in the MAPK/ERK and the PI3K/AKT pathways illustrates its importance in transmitting signals from cell surface receptors. These pathways control a variety of cellular functions including growth survival and metabolism. HRAS interacts with related proteins like KRAS and NRAS integral members of the RAS family. These interactions highlight its role in signaling cascades that contribute to the cellular response to growth factors.
Mutations and aberrant activation of GTPase HRAS link to oncogenic processes particularly in bladder and thyroid cancers. The mutated HRAS protein contributes to the unchecked cellular proliferation seen in tumorigenesis. Additionally HRAS mutations associate with developmental disorders such as Costello syndrome where aberrant signaling disrupts normal developmental processes. HRAS-related pathways often involve other RAS family members like KRAS underlining their collective impact in disease states.