Abcam, ab27043, Anti-58K Golgi protein antibody [58K-9] - Golgi Marker

Size: 100µg
Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) is a mouse monoclonal antibody detecting 58K Golgi protein in Western Blot, Flow Cytometry (Intra), Flow Cytometry . Suitable for Human, Rat . - Over 60 publications - Trusted since 2006
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:58K-9,
Isotype:IgG1,
Carrier free:No,
Reacts with:Rat, Human,
Applications:Flow Cyt (Intra), ICC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-58K Golgi protein antibody [58K-9] - Golgi Marker (ab27043) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human, Rat samples.
What is the molecular weight of 58K Golgi protein?
Anti-58K Golgi protein [58K-9] - Golgi Marker (ab27043) specifically detects a band for 58K Golgi protein (UniProt: O88618) at a molecular weight of 58kDa.
Trusted by the scientific community
Anti-58K Golgi protein [58K-9] - Golgi Marker (ab27043) was first used in a scientific publication in 2006 and has been cited over 60 times in peer-reviewed journals.
Reviewed by scientists
Anti-58K Golgi protein [58K-9] - Golgi Marker (ab27043) has over 15 independent reviews from customers.
Other related products
We have a range of other formats of antibody clone [58K-9] also available for your convenience: ab27043, HRP -
ab202001
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The 58K Golgi protein also known as Golgi marker protein is a significant component within the Golgi apparatus with a molecular mass of approximately 58 kilodaltons (kDa). This protein is widely expressed in eukaryotic cells and serves as an important marker for studying the structure and function of the Golgi apparatus. The 58K Golgi protein localizes predominantly to the membranes of the Golgi where it plays an integral role in the maintenance of its architecture and function. Researchers often use this protein in Golgi immunofluorescence studies to visualize and track Golgi dynamics and morphology.
Biological function summary
The 58K Golgi protein contributes to the Golgi's role in modifying sorting and packaging proteins and lipids for secretion or delivery to other organelles. It does not function alone but often interacts with other Golgi markers and proteins forming part of a complex that helps regulate intracellular transport processes. These interactions are important for maintaining Golgi function and cellular protein distribution. By acting as a Golgi biomarker the 58K Golgi protein aids in understanding the trafficking and processing pathways essential for cellular function.
Pathways
The 58K Golgi protein is involved in the vesicular transport pathway which is essential for trafficking proteins between the endoplasmic reticulum (ER) and the Golgi apparatus. It interacts with other proteins like COPI and COPII which are critical for vesicle formation and movement through this pathway. This protein also plays a role in the glycosylation pathway where nascent proteins undergo carbohydrate modification in the Golgi. Such modifications assist in protein folding stability and cell signaling. The integration of 58K Golgi protein in these pathways highlights its importance in sustaining cellular operations.
The 58K Golgi protein has correlations with some pathologies associated with Golgi dysfunction including congenital disorders of glycosylation and neurodegenerative diseases such as Alzheimer's disease. Disruptions in Golgi structure and function can lead to improper protein sorting and trafficking which is observed in these disorders. Proteins such as amyloid precursor protein (APP) in Alzheimer's are impacted by disruptions in the Golgi pathway where 58K Golgi protein plays a role. Understanding these connections can help in developing therapeutic strategies targeting Golgi-associated dysfunctions.