Abcam, ab270474, Human NEK9 knockout A-431 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
NEK9 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9; X = 10 bp deletion, 2 bp deletion; Frameshift: 99.78%. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A-431,
Species or organism:Human,
Tissue:Skin,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Western blot,
Mutation description:Knockout achieved by CRISPR/Cas9; X = 10 bp deletion, 2 bp deletion; Frameshift: 99.78%,
Disease:Epidermoid Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NEK9, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NEK9 also known as NIMA-related kinase 9 is a serine/threonine kinase with a molecular mass of approximately 118 kDa. It plays a role in the regulation of various cell cycle processes. NEK9 is active in both the cytoplasm and nucleus and expresses broadly in various tissues including the liver kidneys and pancreas. It also has homologs in other eukaryotic organisms indicating its conserved function across species.
Biological function summary
This protein modulates different aspects of mitosis particularly in spindle formation and chromosome segregation. NEK9 forms a part of a complex in conjunction with other kinases like NEK6 and NEK7 coordinating the early events of mitosis. Its activity supports centrosome separation and microtubule nucleation which are important for proper cell division.
Pathways
NEK9 is involved in the cell cycle and microtubule organization pathways. It works in conjunction with proteins such as PLK1 and Aurora A which regulate the entry and progression through mitosis. NEK9 activates NEK6 and NEK7 which further propagate signals necessary for mitotic spindle assembly ensuring efficient cell division.
NEK9 has links to cancer and neurological disorders. Abnormal NEK9 activity has associations with tumorigenesis particularly linked with the dysregulation of cell cycle checkpoints and malignant transformation. In neurological disorder contexts NEK9 interacts with proteins like tau perturbing normal cellular functions and potentially contributing to disease pathology.