Abcam, ab270844, Recombinant Human coronavirus SARS spike glycoprotein (Tagged)

Size: 1mg
Recombinant Human coronavirus SARS spike glycoprotein (Tagged) is a SARS-CoV-2 Fragment protein, expressed in Escherichia coli, with >95%, suitable for ELISA, WB, SDS-PAGE.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Escherichia coli,
Tags:GST tag C-Terminus,
Applications:WB, ELISA, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P0DTC2,
Animal free:No,
Carrier free:No,
Species:SARS-CoV-2,
Storage buffer:Constituents: 50% Glycerol (glycerin, glycerine), 0.4% Tris HCl, 0.4% Sodium-N-Lauroylsarcosinate, 0.25% Triton X-100

Product details:
Contains GST fusion partner. Immunoreactive with SARS positive sera.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SARS spike glycoprotein also known as the S glycoprotein is a critical component of the SARS-CoV-2 virus. It weighs approximately 180 kDa and can be found on the surface of the viral envelope. The spike glycoprotein facilitates the entry of the virus into host cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor. This protein is often targeted by monoclonal antibodies and vaccine strategies aimed at preventing infection. Researchers isolate antibodies that bind specifically to the spike contributing to the development of therapeutic agents like anti-spike monoclonal antibodies.
Biological function summary
The spike glycoprotein serves a fundamental role in the viral life cycle by mediating fusion between the viral membrane and host cell membranes. Its function is divided into two subunits: S1 which is responsible for receptor binding and S2 which is important for membrane fusion. Upon receptor binding a conformational change in the glycoprotein occurs triggering fusion events critical for viral entry. This protein is not typically part of a larger complex but operates in tandem with viral envelope proteins to facilitate replication.
Pathways
The spike glycoprotein's interaction with the host cell's ACE2 receptor places it within the renin-angiotensin-aldosterone system (RAAS) affecting several downstream signaling pathways. The binding initiates endocytosis of the virus into the host cell and impacts inflammation pathways and immune response mechanisms involving interleukin proteins. The spike protein shares functional relationships with other viral proteins such as nucleocapsid and membrane proteins contributing to the virion's structure and infection process.
The spike glycoprotein is closely associated with COVID-19 and the disease's ability to spread and mutate. It's a central focus in the development of potential treatments and vaccines given its role in viral entry. Some mutations in the spike protein can enhance transmissibility or resistance to neutralization influencing the course of infection. In seeking therapeutic interventions researchers focus on spike-related interactions with host proteins like ACE2 which are important in managing COVID-19 symptoms and complications.