Abcam, ab270890, Human ATGL ELISA Kit

Size: 1 x 96Tests
Human ATGL ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ATGL with a sensitivity of 40.78 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture extracts,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 40.78 pg/mL,
Range:218.75 - 14000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human ATGL ELISA kit (ab270890) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ATGL protein in human cell and tissue cullture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human ATGL with 40.78 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human Adipose Triglyceride Lipase (ATGL) catalyzes the initial step in triglyceride hydrolysis in both adipocyte and non-adipocyte lipid droplets. ATGL regulates adiposome size and may be involved in the degradation of adiposomes. ATGL exhibits acylglycerol transaclase activity and may coordinate with LIPE/HLS within the lipolytic cascade. ATGL is also thought to play an important role in energy homeostasis and a role in the response of the organism to starvation. ATGL enhances the hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. ab270890 was designed against the luminal fragment of the ATGL protein. Human ATGL protein sequence identity as follows: mouse, rat, cow, monkey are 90%, 90%, 84% and 92%, respectively.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Adipose Triglyceride Lipase often referred to as ATGL is an important enzyme in lipid metabolism. Also known as lipase 29 or desnutrin ATGL hydrolyzes triglycerides into diglycerides and free fatty acids the first step in the breakdown of stored fat. The molecular weight of the ATGL protein is approximately 54 kDa. Cells express ATGL abundantly in adipose tissues but it also appears in cardiac and skeletal muscles highlighting its importance in energy management across different tissues.
Biological function summary
ATGL is part of the lipid mobilization process and contributes significantly to energy homeostasis. It acts distinctively as it breaks down phosphotriglycerides and is not part of a larger complex carrying out its function independently. Its activity impacts the balance of lipid storage and release important for maintaining energy levels within cells and across the entire organism.
Pathways
ATGL plays a significant role in the lipid catabolism and energy regulation pathways. ATGL's activity regulates the fat mobilization pathway by interacting closely with the protein Comparative Gene Identification-58 (CGI-58) which activates it. Further ATGL is involved in the peroxisome proliferator-activated receptor (PPAR) pathway highlighting its role in influencing metabolic processes and energy balance.
ATGL dysregulation connects to metabolic disorders such as obesity and non-alcoholic fatty liver disease (NAFLD). Reduced ATGL activity leads to excessive triglyceride accumulation in adipose tissue contributing to the development of obesity. Moreover ATGL deficiencies can link to cardiac issues through its connection with another lipase Hormone-Sensitive Lipase (HSL) affecting lipid content in cardiac tissues and influencing cardiomyopathies. Understanding ATGL's precise function aids in the development of therapeutic strategies for these conditions.