Abcam, ab270897, Human GBP1 ELISA Kit

Size: 1 x 96Tests
Human GBP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human GBP1 with a sensitivity of 24.09 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 24.09 pg/mL,
Range:109.38 - 7000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human GBP1 ELISA kit (ab270897) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GBP1 protein in human citrate and heparin plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate human GBP1 with 24.09 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human Guanylate-binding protein 1 (GBP1) is a 67 kDa GTPase. Protein expression is induced by response to interferon gamma. GBP1 hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. It has been reported to have antiviral activity against the influenza virus. In addition, GBP1 has been shown to promote oxidative killing and delivery of antimicrobial peptides the autophagolysosomes, providing broad host protection against different pathogen classes. Human GBP1 shares 69%, 72%, 22% sequence identity with mouse, rat, and cow, respectively.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target known commonly as GBP1 or guanylate-binding protein 1 plays a mechanical role as a large GTPase with a molecular mass of approximately 67 kDa. This protein is expressed in various tissues most notably in interferon-gamma-induced human cells. GBP1 localizes mainly in the cytoplasm and associates with cellular membranes. Alternate designations for the GBP1 protein include GBP 1. Its role as a GTPase involves hydrolyzing GTP which is critical for its cellular functions.
Biological function summary
The GBP1 protein supports cellular defense mechanisms against pathogens such as viruses and bacteria. GBP1 participates in the hydrolysis of GTP to GMP an action that influences its anti-pathogenic effects. It is a member of the interferon-inducible large GTPase family and can form oligomeric complexes enhancing its antiviral and antimicrobial activity. It interacts with various proteins linked to innate immunity and contributes to modulating the immune response.
Pathways
GBP1 is involved in pathways linked to interferon signaling and innate immunity. The protein plays a significant role in the JAK-STAT signaling pathway where it exerts its antiviral effects. GBP1 interacts closely with other proteins such as STAT1 and is responsive to interferon signals influencing gene expression and immune responses. Its activity in these pathways is critical for mounting an effective defense against infections.
GBP1 has been associated with inflammatory diseases and cancer. The protein's expression levels often increase in response to inflammatory signals implicating it in the pathogenesis of diseases like Crohn's disease. Moreover GBP1 has a connection with cancer-related mechanisms where it might affect tumor cell proliferation. In these contexts proteins such as STAT1 and other interferon-responsive proteins relate closely to the function and regulatory mechanisms of GBP1.