Abcam, ab271075, Anti-NOP2 antibody [EPR24888-92]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal NOP2 antibody. Suitable for ICC/IF, IP, Flow Cyt (Intra), WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 5 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24888-92,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Mouse, Human,
Applications:WB, IHC-P, IP, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NOP2 also known as NOP2 nucleolar protein is a methyltransferase enzyme involved in ribosomal RNA (rRNA) processing. It weighs approximately 82 kDa. NOP2 is widely expressed in various cell types particularly in rapidly dividing cells where ribosome biogenesis is active. The protein resides predominantly in the nucleolus where it assists in rRNA modification and proper ribosome assembly.
Biological function summary
NOP2 plays an important role in the maturation of 28S rRNA during the biogenesis of the 60S ribosomal subunit. It functions as part of the larger ribosome assembly complex. By modifying rRNA NOP2 ensures correct rRNA folding and stabilization essential for the production of functional ribosomes. This enzymatic activity aligns with its critical role in the proliferation of cells impacting processes such as protein synthesis and cell growth.
Pathways
NOP2 is intimately involved in the ribosome biogenesis and assembly pathway. It interacts with other ribosomal proteins and ribosome assembly factors such as NOP58 during ribosome maturation. NOP2's role extends into the mitotic cell cycle pathway where it influences cellular proliferation. Understanding its connection with these pathways supports insights into cellular growth regulation and transformation.
NOP2 associates strongly with cancer pathogenesis. Overexpression of NOP2 frequently occurs in various cancers suggesting a potential role in oncogenesis. Dysregulated ribosome biogenesis facilitated by NOP2 can lead to tumor progression and metastasis. The protein's interactions with oncoproteins such as c-Myc further emphasize its importance in understanding cancer mechanisms and therapeutic targets.