Abcam, ab271358, Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active)

Size: 10µg
Recombinant human Acetyl Coenzyme A Carboxylase beta protein (Active) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>80% SDS-PAGE,
Expression system:Baculovirus infected Sf9 cells,
Tags:His tag C-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific Activity: ≥20 pmoles/min/μgAssay Conditions: 25 μl Acetyl Coenzyme A Carboxylase beta reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl2, 2 mM potassium citrate, 1 mM DTT, 100 μM ATP, 12 mM NaHCO3 and 20 μM acetyl-CoA at 30°C for 45 min. ATP reduction is detected.,
Accession:O00763,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Preservative: 0.68% ImidazoleConstituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Acetyl Coenzyme A Carboxylase beta (ACCβ) also known as ACC2 is an enzyme that catalyzes the conversion of acetyl-CoA to malonyl-CoA. This enzyme has a molecular mass of about 280 kDa. Mechanically ACCβ is important in initiating fatty acid biosynthesis. ACCβ is expressed most abundantly in skeletal muscle liver and heart tissues. These tissues have a significant demand for fatty acid metabolism and energy regulation.
Biological function summary
The enzyme controls fatty acid oxidation by regulating malonyl-CoA levels which in turn inhibits carnitine palmitoyltransferase 1 (CPT1) in the mitochondrial membrane. This action helps balance the partitioning between fatty acid synthesis and oxidation. ACCβ functions not in isolation but as part of a larger complex that can undergo polymerization or depolymerization impacting its activity. ACCβ impacts energy homeostasis by influencing cellular energy stores and metabolic flux.
Pathways
ACCβ plays a significant role in the fatty acid metabolism and insulin signaling pathways. It is a pivotal enzyme in the control of lipid biosynthesis and oxidation balance. Key proteins related to these pathways include AMP-activated protein kinase (AMPK) which phosphorylates and inactivates ACCβ therefore modulating its activity in response to energy levels. This regulation integrates nutritional signals with energy metabolism.
ACCβ is linked to metabolic disorders such as obesity and type 2 diabetes. Alterations in ACCβ function can lead to dysregulation of lipid metabolism contributing to these conditions. Also through its role in fatty acid metabolism ACCβ has connections to diseases mediated by CPT1 dysregulation potentially affecting heart disease and other lipid-associated disorders where metabolic imbalance plays a role.