Abcam, ab271887, Anti-CD168 antibody [EPR4054] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CD168 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR4054,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IP, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab271887 is the carrier-free version of
ab124729
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD168 also known as HMMR (hyaluronan-mediated motility receptor) is a protein that functions mechanically as a receptor involved in cell motility proliferation and adhesion. Its molecular weight is approximately 95 kDa. CD168 expression can be prominently found in tissues that contain a high density of hyaluronan such as tumor cells and certain types of epithelial cells. Additionally CD168 is often used as a marker for certain macrophage populations illustrating its role in the immune landscape.
Biological function summary
Hyaluronan-mediated motility receptor plays an important role in the regulation of the extracellular matrix (ECM) by binding to hyaluronan a significant component of the ECM. CD168 has a vital function in cell surface signaling processes facilitating communication between cells and their environment. The protein operates in a complex with elements like growth factor receptors enhancing its ability to modulate cellular responses. This interaction allows CD168 to significantly influence processes like wound healing and tissue remodeling.
Pathways
Hyaluronan-mediated motility receptor participates in the Rho signaling pathway important for cytoskeletal organization and cell movement. Through this pathway CD168 interacts directly with proteins such as GTPases including members of the Rho family like RhoA which play key roles in the reorganization of the actin cytoskeleton. CD168 also relates to the PI3K/AKT pathway influencing cellular survival and proliferation by interacting with proteins such as PI3K integrally involved in cellular growth and response to extracellular signals.
Increased expression of hyaluronan-mediated motility receptor has been observed in several cancers including breast and ovarian cancers where it often correlates with advanced disease stages and poor prognosis. CD168's role in cancer is linked with its interaction with the epidermal growth factor receptor (EGFR) which promotes aggressive tumor behavior. In arthritis CD168 expression contributes to synovial inflammation connecting with inflammatory cytokines that escalate the disease progression. These associations underline the importance of CD168 in both oncology and inflammatory disorders.