Abcam, ab272050, Anti-FOXL2 antibody [EPR23523-68] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal FOXL2 antibody. Carrier free. Suitable for IP, WB, IHC-Fr, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23523-68,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IP, IHC-Fr, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab252050
is the carrier-free version of
ab246511
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein FOXL2 also known as Forkhead box L2 functions as a transcription factor belonging to the forkhead family. It has an approximate mass of 45 kDa. This protein is expressed mainly in ovarian somatic cells and certain tissues of the eye. FOXL2 contains a distinct forkhead domain which binds to DNA and regulates gene expression involved in cellular proliferation differentiation and apoptosis.
Biological function summary
Cells rely on FOXL2 for regulating early ovarian development and maintenance of granulosa cell identity. As part of transcriptional complexes it interacts with other proteins to maintain ovarian function and health. FOXL2 also inhibits certain genes that are otherwise potentially involved in abnormal cell growth acting as a tumor suppressor in ovarian tissues. This makes it essential in reproductive biology.
Pathways
FOXL2 plays significant roles in ovarian development and function through the TGF-beta signaling pathway and the Wnt signaling pathway. In collaboration with SMAD3 a protein in the TGF-beta pathway FOXL2 regulates genes important for cell communication and differentiation. Additionally it interfaces with proteins involved in cellular structure and movement influencing biological processes critical for tissue maintenance.
Mutations in FOXL2 are linked to disorders like Blepharophimosis Ptosis Epicantherefore Inversus Syndrome (BPES) and ovarian granulosa cell tumors. FOXL2 mutations often disrupt the protein’s interactions with other factors such as the androgen receptor leading to abnormal cellular function. In BPES FOXL2 mutations impair eyelid formation and function highlighting its role in craniofacial development.