Abcam, ab272073, Anti-PBRM1/BAF180 antibody [BL-39-2C3] - BSA free

Size: 100µL
Rabbit Recombinant Monoclonal PBRM1/BAF180 antibody. Suitable for IHC-P, ICC, IP, WB, Flow Cyt and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PBRM1 aa 1-300.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:BL-39-2C3,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WB, IHC-P, Flow Cyt, ICCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PBRM1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.Q86U86

Product details:
ab272073 is the BSA-free version of
ab243876
This product is sold under License from Bethyl Laboratories, Inc.

Properties and Storage Information:
Form-Liquid, Purification notes-Recombinant antibody was purified from cell culture supernatant., Storage buffer-pH: 8.2Preservative: 0.09% Sodium azideConstituents: 98% Borate buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PBRM1 also known as BAF180 is an important component of the SWI/SNF chromatin remodeling complex. It has a molecular weight of approximately 187 kDa. Within the cell PBRM1 is expressed in various tissues but shows higher expression in kidney and breast tissues. Its role involves binding to acetylated histones which influences chromatin structure and gene expression regulation.
Biological function summary
The PBRM1/BAF180 protein functions as part of the SWI/SNF complex which includes the catalytic subunit BRG1 or BRM. This complex is important for altering chromatin architecture which regulates access of transcriptional machinery to DNA. It plays a significant role in the control of cell growth and differentiation processes. The structural integrity of PBRM1 is necessary for the functionality of the SWI/SNF complex.
Pathways
PBRM1/BAF180 is an important player in pathways involving chromatin remodeling and transcriptional regulation. It is implicated in the WNT signaling pathway interacting with proteins such as β-catenin to influence gene transcription. It also partakes in the p53 pathway where it contributes to the regulation of cell cycle and apoptosis by modulating p53 activity.
Mutations in PBRM1 are frequently observed in renal cell carcinoma. Its disruption affects chromatin remodeling which disrupts normal gene expression contributing to cancer progression. PBRM1 also relates to breast cancer where alterations can promote tumor formation. In these contexts the interaction of PBRM1 with other proteins like VHL in renal carcinoma helps elucidate its role in tumor suppression and development.