Abcam, ab272174, Anti-GALC antibody [EPR23598-126] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GALC antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23598-126,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab272174 is the carrier-free version of
ab240638
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GALC also known as galactocerebrosidase or galactosylceramidase is an enzyme with a molecular mass of about 68 kDa. It functions mechanically by hydrolyzing galactosylceramide (galactocerebroside) into galactose and ceramide. GALC expresses widely in the brain and other nervous tissues particularly in the lysosomes of glial cells contributing to the breakdown of lipids. The efficient lysosomal degradation process performed by GALC is critical for maintaining cellular lipid homeostasis.
Biological function summary
GALC plays an important role in the metabolism of complex lipids. It functions not as part of a larger protein complex but rather as an independent enzyme acting on specific substrates like galactosylceramide. The enzyme's activity contributes to the transformation of lipids necessary for the proper structure and function of myelin in the central nervous system. By maintaining proper lipid levels GALC supports the integrity and stability of myelinated neurons.
Pathways
GALC is involved in the sphingolipid degradation pathway contributing to the catabolic process of sphingolipids. This pathway ensures the break down and recycling of sphingolipid components like ceramide which are essential for cell signaling and membrane integrity. Another relevant pathway is the lysosomal storage pathway where GALC's enzymatic function prevents the abnormal accumulation of galactosylceramide and related substrates. GALC activity has links to other lysosomal enzymes like arylsulfatase A which also participate in lipid metabolism within the lysosome.
GALC mutations and deficiencies lead to Krabbe disease a severe lysosomal storage disorder affecting the nervous system. This pathology results from the accumulation of psychosine a toxic metabolite causing widespread demyelination. Another related condition is globoid cell leukodystrophy associated with GALC dysfunction. Proteins like myelin-associated glycoprotein may interact indirectly with GALC through these disease processes as GALC's enzymatic activity affects myelin maintenance.