Abcam, ab272237, Anti-GATA2 + GATA3 antibody [EPR24359-3]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal GATA2 antibody. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24359-3,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Rat, Mouse,
Applications:WB, IP, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GATA3 and GATA2 are transcription factors that belong to the GATA family known for their role in DNA binding and regulating gene expression. GATA3 also called GATA-binding protein 3 has a molecular mass of around 50 kDa. These factors are expressed in various tissues but show high expression levels in haematopoietic cells T lymphocytes and epithelial cells. GATA proteins contain two highly conserved zinc finger domains that are essential for binding to the DNA sequence A/T-GATA-A/G influencing the transcription of genes involved in cellular differentiation and proliferation.
Biological function summary
These GATA proteins play critical roles in the development and differentiation of several cell types. GATA3 for instance is essential in T-cell differentiation and function dictating the fate of Th2 lymphocytes and influencing cytokine production. It is also involved in the development of epithelial cells in mammary glands. Both GATA2 and GATA3 may interact with other proteins and form transcriptional complexes impacting a wide range of biological processes. Their ability to regulate gene expression makes them key to proper immune system functionality and tissue homeostasis.
Pathways
GATA3 and GATA2 participate significantly in maintaining the balance of the immune response and cellular proliferation. GATA3 features importantly in the Th2-cell immune response pathway where it regulates genes necessary for this pathway's effective functioning. It relates to the STAT6 signaling pathway where it works in concert with proteins like IL-4 to stimulate Th2 differentiation. GATA2 on the other hand associates with the hematopoietic stem cell renewal and differentiation pathway interacting with FOG1 and PU.1 proteins to regulate these processes.
These transcription factors have implications in various pathologies. Dysregulation of GATA3 is closely linked with allergic responses and autoimmune diseases such as asthma through its regulation of Th2 cytokines. It also connects to breast cancer where abnormal GATA3 expression influences tumorigenesis involving other proteins like ERBB2. GATA2 mutations associate with hematologic disorders such as myelodysplastic syndromes highlighting their impact on blood cell differentiation and maturation. Understanding these associations provides insight into potential therapeutic targets for these diseases.