Abcam, ab272354, Anti-RFC antibody

Size: 100µg
Rabbit Polyclonal RFC antibody. Suitable for WB and reacts with Mouse, Rat, African green monkey, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC19A1 aa 200-250.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human, African green monkey,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SLC19A1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.P41440

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Preservative: 0.025% Sodium azideConstituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate, dihydrate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Reduced Folate Carrier (RFC) also known as SLC19A1 functions as a bidirectional anion exchange transporter which facilitates the transport of folate and antifolate compounds across cellular membranes. This protein has a molecular mass of approximately 65 kDa. RFC achieves high expression levels in hematopoietic tissues the placenta and the choroid plexus indicating its critical role in tissues where folate demands are significant. Additionally its distribution in various cell types suggests it plays an essential role in folate homeostasis across the body.
Biological function summary
RFC operates as an important component of a membrane transport system that influences cellular folate uptake. It ensures an adequate supply of folate a vital nutrient for one-carbon metabolism linked to DNA synthesis repair and methylation processes. RFC functions independently not as a part of any larger protein complex but interacts with other cellular systems to maintain folate levels. Its activity significantly affects cellular proliferation because folate acts as a donor of methyl groups necessary for thymidine and purine biosynthesis critical precursors for DNA production.
Pathways
Folate transport by RFC supports essential pathways such as the tetrahydrofolate cycle and the methionine cycle. These pathways are indispensable for the synthesis of nucleotides and the regeneration of homocysteine to methionine impacting processes like DNA methylation and gene expression. In these pathways RFC operates in conjunction with proteins like methionine synthase and dihydrofolate reductase which further facilitate the folate cycle showing the protein's integration into cellular metabolism.
RFC influences conditions like acute lymphoblastic leukemia (ALL) and neural tube defects (NTDs). Altered RFC function or expression can affect folate homeostasis leading to impaired DNA synthesis and repair in rapidly dividing cells which is a characteristic of ALL. Furthermore insufficient folate transport through RFC can result in inadequate neural tube closure during fetal development related to NTDs. Associations with proteins like thymidylate synthase have shown RFC's role in antifolate drug resistance emphasizing its impact on cancer therapeutics.