Abcam, ab272367, Anti-AKT1 (pS473) + AKT2 (pS474) + AKT3 (pS472) antibody [EPR18853] - BSA and Azide free (Detector)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal AKT1 phospho S473 antibody. Carrier free. Suitable for sELISA, Dot and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR18853,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Dot, sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab272367 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human AKT3 (pS473). The recommended pair for sandwich ELISA is:
Capture:
ab242892
, Human AKT3 (pS473) Capture Antibody (unconjugated)
Detector: ab272367, Human AKT3 (pS473) Detector Antibody (unconjugated)
The reference range value is 46.88 - 3000 U/ml.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein kinase AKT also known as protein kinase B (PKB) includes three isoforms: AKT1 AKT2 and AKT3. These play a central role in cellular processes. AKT1 has a molecular weight of approximately 56 kDa AKT2 has around 55 kDa and AKT3 similarly weighs about 54 kDa. These isoforms of AKT are expressed in various tissues but AKT1 is abundantly found in most tissues AKT2 is often present in insulin-responsive tissues like skeletal muscle and adipose tissue and AKT3 is mainly in the brain. The activation of AKT involves phosphorylation which increases their kinase activity significantly. The isoforms have unique and overlapping functions offering distinct mechanical roles in cellular signaling.
Biological function summary
The AKT kinase family plays a major role in regulating vital cellular functions including metabolism cell proliferation survival and growth. It does so by catalyzing phosphorylation of a range of substrates within these pathways. AKT is often involved in complexes with other proteins to achieve these biological effects. It impacts the regulation of glycogen synthase kinase 3 (GSK3) involved in insulin response and mTOR which controls cell growth. These wide-ranging functions make AKT proteins essential in maintaining cellular homeostasis.
Pathways
AKT proteins are important components of the PI3K/AKT/mTOR signaling pathway and the insulin signaling pathway. Within these pathways they interact with and modulate activities of related proteins such as PI3K PDK1 and mTOR. This regulation helps control the balance between anabolic and catabolic processes influencing cell survival growth and metabolism. The importance of AKT kinases in these pathways marks them as key regulators of cellular responses to external and internal stimuli.
AKT isoforms have strong connections to cancer and diabetes. Over-activation of AKT pathways frequently occurs in cancerous cells contributing to uncontrolled cell growth and survival. In diabetes AKT2 is particularly implicated due to its role in insulin signaling influencing how cells process glucose. Dysregulation of AKT pathways has been linked to the development and progression of these diseases where proteins like mTOR in cancer and IRS1 in diabetes show direct relation to AKT function and malfunctions.