Abcam, ab272400, Anti-C5a-R antibody [EPR23278-117] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal C5a-R antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23278-117,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab272400 is the carrier-free version of
ab252435
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C5a-R also known as CD88 or C5aR1 is a protein found primarily on the surface of immune cells like macrophages and neutrophils. Its mass is approximately 45 kDa. C5a-R functions as a G protein-coupled receptor (GPCR) and binds the complement component C5a a powerful inflammatory peptide. The receptor is expressed in various tissues but primarily in the immune system where it plays a significant role in immune responses and inflammatory processes.
Biological function summary
C5a-R functions as a mediator of inflammation and immune responses. It is a part of the complement system a complex of proteins involved in innate immunity. Upon binding to C5a this receptor initiates a signaling cascade that leads to the recruitment and activation of immune cells at sites of inflammation. It amplifies the inflammatory response leading to cell chemotaxis degranulation and the production of pro-inflammatory cytokines.
Pathways
C5a-R plays an important role in the complement and immune pathways. It interacts closely with the C5 protein which is a core component of the complement pathway. This receptor also connects to other immune system proteins such as the C3 and C4 proteins through the cascade. Together these interactions help defend against pathogenic infections and maintain immune homeostasis.
C5a-R is associated with conditions like rheumatoid arthritis and sepsis. In rheumatoid arthritis the receptor's activation by C5a contributes to chronic inflammation and joint damage. During sepsis overactivation of C5a-R can lead to excessive inflammation and tissue damage. The receptor's involvement in these diseases is often linked with other proteins in the complement system such as C3a and C4a which also play roles in modulating inflammation and immune responses.