Abcam, ab272420, Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus

Size: 50µg
Mouse Monoclonal SPIKE antibody. Suitable for ICC, WB and reacts with SARS-CoV samples. Cited in 23 publications. Immunogen corresponding to Virus preparation containing SARS spike glycoprotein protein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3A2,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:SARS-CoV,
Applications:ICC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:We have received mixed feedback regarding the specificity of this antibody against SARS-Cov2, please see the customer reviews section.

Product details:
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data Reviews: Customers have been successful and shared their AbReview

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 6.5 - 8.5Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SARS spike glycoprotein also known as the S glycoprotein is a critical component of the SARS-CoV-2 virus. It weighs approximately 180 kDa and can be found on the surface of the viral envelope. The spike glycoprotein facilitates the entry of the virus into host cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor. This protein is often targeted by monoclonal antibodies and vaccine strategies aimed at preventing infection. Researchers isolate antibodies that bind specifically to the spike contributing to the development of therapeutic agents like anti-spike monoclonal antibodies.
Biological function summary
The spike glycoprotein serves a fundamental role in the viral life cycle by mediating fusion between the viral membrane and host cell membranes. Its function is divided into two subunits: S1 which is responsible for receptor binding and S2 which is important for membrane fusion. Upon receptor binding a conformational change in the glycoprotein occurs triggering fusion events critical for viral entry. This protein is not typically part of a larger complex but operates in tandem with viral envelope proteins to facilitate replication.
Pathways
The spike glycoprotein's interaction with the host cell's ACE2 receptor places it within the renin-angiotensin-aldosterone system (RAAS) affecting several downstream signaling pathways. The binding initiates endocytosis of the virus into the host cell and impacts inflammation pathways and immune response mechanisms involving interleukin proteins. The spike protein shares functional relationships with other viral proteins such as nucleocapsid and membrane proteins contributing to the virion's structure and infection process.
The spike glycoprotein is closely associated with COVID-19 and the disease's ability to spread and mutate. It's a central focus in the development of potential treatments and vaccines given its role in viral entry. Some mutations in the spike protein can enhance transmissibility or resistance to neutralization influencing the course of infection. In seeking therapeutic interventions researchers focus on spike-related interactions with host proteins like ACE2 which are important in managing COVID-19 symptoms and complications.