Abcam, ab272596, Anti-PRPF3 antibody

Size: 100µL
Rabbit Polyclonal PRPF3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PRPF3 aa 350-500.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human PRPF3 aa 350-500. The exact immunogen used to generate this antibody is proprietary information.O43395

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRPF3 also known as Pre-mRNA processing factor 3 plays an important role in the spliceosome machinery necessary for the splicing of pre-mRNA. It is part of the U4/U6-U5 tri-snRNP complex which is important for the proper assembly of the spliceosome. This protein weighs approximately 77 kDa and can be found in the nucleus of cells where mRNA splicing takes place. Its expression occurs across various tissues reflecting its relevance in general cellular functions.
Biological function summary
PRPF3 is involved in mRNA processing by facilitating the removal of introns from pre-mRNA transcripts. In its role within the spliceosome it works alongside other proteins in the complex to ensure accurate intron excision and exon ligation. This process is essential for creating mature and functional mRNA which translates into proteins vital for cellular operations. PRPF3 ensures the precision of RNA splicing affecting gene expression regulation and influencing alternative splicing events.
Pathways
PRPF3 is integrated into the spliceosomal cycle and is essential for the regulation of gene expression. This protein interacts with other spliceosome-associated proteins such as PRPF8 and SNRNP200 participating in the assembly and activation of the spliceosome. Its function influences the mRNA surveillance pathway ensuring only properly spliced mRNAs are translated into proteins which is a critical regulatory mechanism in cells.
Mutations in the PRPF3 gene have been linked to Retinitis Pigmentosa 18 a genetic disorder leading to progressive vision loss. The dysfunction can impact the interactions of PRPF3 with other spliceosomal proteins such as PRPF8 disrupting normal RNA splicing and leading to photoreceptor cell death. Additionally aberrant splicing events involving PRPF3 can contribute to other genetic conditions where precise splicing is impaired highlighting its importance in maintaining cellular homeostasis.