Abcam, ab272608, Anti-TRPML1/MG-2 antibody

Size: 100µL
Rabbit Polyclonal TRPML1/MG-2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCOLN1 aa 500 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MCOLN1 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9GZU1

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The TRPML1 also known as mucolipin-1 or MG-2 is a member of the transient receptor potential (TRP) channels family. This protein functions primarily as a Ca\(^2+\)/H\(^+\) exchange channel across the lysosomal membrane. TRPML1 has a mass of approximately 65 kDa. Researchers have found it in various tissues with notable expression in the kidney heart and liver. It plays an important role in lysosomal trafficking and autophagic flux impacting cellular waste management systems.
Biological function summary
The TRPML1 channel regulates the movement of calcium ions from lysosomes to the cytosol. It performs as part of the TRPML1-3 complex that coordinates lysosome-endosome fusion ensuring lysosomal homeostasis. The channel also impacts lysosome biogenesis and function making it a valuable player in cellular metabolic processes. Its proper function controls several cellular degradative pathways supporting efficient macromolecule turnover.
Pathways
TRPML1 participates significantly in autophagy and endocytosis pathways. It partners with proteins such as mTOR and TFEB regulating lysosomal biogenesis and nutrient sensing. Through these interactions TRPML1 adjusts the cellular response to nutrient deprivation and stress promoting survival when nutrients are scarce. Its interaction with other TRP channels enhances lysosomal calcium regulation integrating with broader metabolic and catabolic processes.
Mutations or dysfunctions in the TRPML1 gene are closely linked to mucolipidosis type IV a severe neurodegenerative disorder. The channel's malfunction disrupts lysosomal function leading to the buildup of storage materials in cells. Additionally TRPML1 abnormalities have connections to Parkinson’s disease due to its role in autophagy and cellular clearance. The protein's interaction with LRRK2 another protein associated with Parkinson’s highlights its relevance in neurodegenerative pathways offering potential therapeutic targets for treatment.