Abcam, ab273151, Human IgG ELISA kit - 30 minutes

Size: 1 x 96Tests / 10 x 96Tests
Human IgG ELISA kit - 30 minutes is a sandwich ELISA designed to quantify human IgG with a sensitivity of 4 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Rapid and easy method - 30 min protocol - Wide dynamic range - quantifies 16 - 1000 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum,
Assay type:Quantitative,
Sensitivity:= 4 ng/mL,
Range:16 - 1000 ng/mL,
Assay Platform:Microplate

Product details:
The Human IgG quantification Kit (ab273151) provides a rapid and easy method (one step ELISA) for the quantitative determination of human IgG in cell culture supernatant and serum. The kit includes ready-to-use reagents necessary to analyze 89 samples in less than 30 minutes.
A polyclonal antibody specific to the gamma heavy chain of human IgG is pre-coated onto microwells. Samples and standards are pipetted into microwells and human IgG present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-human IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of human IgG in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C