Abcam, ab273221, Porcine Decorin ELISA Kit

Size: 1 x 96Tests
Porcine Decorin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Porcine Decorin in Human, Pig in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum,
Reacts with:Human, Pig,
Assay type:Sandwich (quantitative),
Sensitivity:= 4 pg/mL,
Range:4.1 - 1000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Porcine Decorin ELISA Kit 9ab273221)) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine Decorin in serum, plasma (Collect plasma using EDTA and citrate as an anti-coagulant.
Heparin is not recommended
as anti-coagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for porcine Decorin coated on a 96-well plate. Standards and samples are pipetted into the wells and Decorin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-porcine Decorin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Decorin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C