Abcam, ab273286, Protein Synthesis Assay Kit (Green, Microplate)

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Protein Synthesis Assay Kit (Green, Microplate) (ab273286) labels cells undergoing translation with a fluorescent green dye, using a method based on an alkyne analog of puromycin; O-Propargyl-puromycin (OP-puro). Readout on a fluorometric plate reader. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Quantitative,
Assay Platform:Microplate

Product details:
Protein Synthesis Assay Kit (Green, Microplate) (ab273286) utilizes a novel and robust chemical method based on an alkyne-containing, cell-permeable analog of puromycin, O-Propargyl-puromycin (OP-puromycin).
Protein synthesis assay principle
In the protein synthesis assay, cultured cells are incubated with OP-puromycin. Once inside the cell, OP-puromycin stops translation by forming covalent conjugates with nascent polypeptide chains.
After incubation, cells are then fixed with formalin, washed, and permeabilized with detergent.
Truncated polypeptides bound to OP-puromycin are then labelled with a fluorescent dye, in a copper-dependent reaction using a fluorescent-azide.
Cycloheximide, an inhibitor of translation, is used as a negative control. Sytox Green is used as a nuclear counter-stain.
Unlike methionine analogs, OP-puromycin does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture.
Other protein synthesis assay kits
This kit ab273286 stains cells that have undergone protein synthesis with 5(6)-FAM (Fluorescein), which has a green fluorescence. It is designed for analysis by fluorometric microplate reader.
This kit contains identical components to Protein Synthesis Assay Kit (Green)
ab239725
which is designed for analysis by fluorescent microscopy or flow cytometry.
Protein Synthesis Assay Kit (Red)
ab235634
stains cells that have undergone protein synthesis with 5(6)-TAMRA (Rhodamine), which has a red fluorescence (Ex//Em 540/580 nm). It is designed for analysis by fluorescent microscopy or flow cytometry.
Other Notes
This product was previously called K467 Biovision EZClick™ Global Protein Synthesis Assay Kit and Global Protein Synthesis Assay Kit. Biovision acquired Abcam in 2021.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C