Abcam, ab273439, Anti-CRISPR protein AcrIIA4 antibody [7C5-3A8]

Size: 20µg / 100µg / 1mg
Mouse Recombinant Monoclonal Anti-CRISPR protein AcrIIA4 antibody. Suitable for WB and reacts with Transfected cell line - Bacteriophage sp. samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:7C5-3A8,
Isotype:IgG1,
Carrier free:No,
Reacts with:Bacteriophage sp.,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Species reactivity: Bacteriophage sp.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Anti-CRISPR protein AcrIIA4 also known simply as AcrIIA4 acts as a potent inhibitor of the CRISPR/Cas9 system. This protein binds to the Cas9 enzyme preventing it from associating with DNA and stopping the gene-editing process. AcrIIA4 has a molecular mass of approximately 12 kDa and is expressed in various bacterial species most notably in Listeria monocytogenes. The mechanical inhibition of the CRISPR activity by AcrIIA4 is specific targeting the Cas9 DNA-binding groove to block its function effectively.
Biological function summary
This anti-CRISPR protein plays a role in bacterial defense against phages that use CRISPR/Cas systems to target bacterial cells. AcrIIA4 operates as part of a protein complex within the bacterial cell working alongside other cell machinery to ensure proper functioning of bacterial genes without interference from CRISPR systems. By blocking the CRISPR/Cas mechanism AcrIIA4 can maintain bacterial genome stability in environments where genetic modification pressure is high.
Pathways
AcrIIA4 participates in bacterial immune evasion pathways notably interfering with the CRISPR-associated immune pathway. The most important pathway related to this is the Type II CRISPR/Cas system where AcrIIA4 directly targets the Cas9 protein. This interaction prevents the cleavage of target DNA by the Cas9 complex offering insight into regulatory mechanisms that control gene-editing processes in both natural and synthetic settings. AcrIIA4 connects with other anti-CRISPR proteins which together modulate the overall impact of CRISPR activity in microbial communities.
AcrIIA4's primary implications relate to bacterial pathogenicity and potential impacts on antimicrobial resistance. Understanding AcrIIA4 in pathogens like Listeria monocytogenes helps unravel its role in microbial resistance to phage therapies which might be used to treat bacterial infections. Additionally as the inhibition mechanism can affect gene-editing efficiency AcrIIA4 is relevant in discussions about potential off-target impacts in CRISPR therapeutic applications mediated through Cas9 misregulation.