Abcam, ab273879, Anti-Adenosine Receptor A2a antibody [EPR23731-50] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Adenosine Receptor A2a antibody. Carrier free. Suitable for IHC-P and reacts with Mouse, Human, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23731-50,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Expression levels of the target protein vary with sample type and optimisation may be required. In our hands we are only able to detect ADORA2A in relevant neurological samples and do not recommend this product for use in other samples.

Product details:
ab273879 is the carrier-free version of
ab260032
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Adenosine Receptor A2A also known as A2AR is a G protein-coupled receptor with a molecular mass of approximately 45 kDa. This receptor is widely expressed in various body tissues including the brain heart spleen and immune cells. A2A receptors play a mechanical role in mediating physiological responses to adenosine a purine nucleoside that acts as a signaling molecule in the body. Through binding to adenosine A2A receptors activate intracellular pathways that modulate cellular responses.
Biological function summary
The A2A adenosine receptor functions as an important regulator of neurotransmission and immune response. It does not form part of a larger protein complex but rather independently influences processes such as vasodilation heart rate regulation and cytokine release. The A2A receptor contributes to the modulation of dopamine and glutamate signaling in the central nervous system playing a significant role in maintaining neural balance. Its activity in immune cells helps in controlling inflammation and immune cell proliferation.
Pathways
The A2A adenosine receptor engages in critical signaling cascades particularly the cAMP-PKA pathway and the MAPK pathway. Through the cAMP-PKA pathway A2A R influences various cellular processes by increasing cyclic AMP levels which activate protein kinase A. The receptor is also involved in the MAPK pathway impacting cellular growth and differentiation. It interacts with proteins such as Gs alpha subunit which couples to the receptor to promote downstream signaling events.
The A2A receptor has connections to conditions like Parkinson's disease and ischemia-reperfusion injury. In Parkinson's disease the activity of A2A receptors affects dopaminergic signaling where it interacts with proteins such as dopamine D2 receptors suggesting potential for therapeutic targeting. During ischemia-reperfusion injury the receptor's modulation of immune responses and vasodilation can influence tissue damage and repair mechanisms linking it with proteins involved in inflammatory pathways.