Abcam, ab274327, Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free

Size: 100µg / 1mg
Knockout Tested Rabbit Recombinant Monoclonal HTRA1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23240-64,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab274327 is the carrier-free version of
ab274322
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein HtrA1 also known as High Temperature Requirement A Serine Peptidase 1 functions mechanically as a serine protease. It has a mass of approximately 51 kDa and plays a critical role in protein quality control by degrading misfolded proteins. HtrA1 is expressed in various tissues including the placenta brain and lung. It localizes to the extracellular region indicating its involvement in extracellular matrix regulation.
Biological function summary
HtrA1 participates in cellular processes including protein degradation and modulation of signaling pathways. It does not have a known direct association with protein complexes but its protease activity affects extracellular matrix components and influences cellular homeostasis. HtrA1's regulatory function can affect cell growth and differentiation impacting tissue development and repair mechanisms.
Pathways
HtrA1 interacts significantly with the TGF-beta signaling pathway where its protease activity modulates the availability and activity of TGF-beta ligands and receptors. It also plays a role in the Wnt signaling pathway by influencing the stability of pathway-related proteins. HtrA1 interacts with proteins such as TGF-beta contributing to the regulation of extracellular matrix and cellular responses to external signals.
HtrA1 has connections to osteoarthritis and age-related macular degeneration (AMD). Elevated expression of HtrA1 correlates with the degeneration of articular cartilage and extracellular matrix breakdown in osteoarthritis. In AMD HtrA1 influences the structural integrity of ocular tissues with disturbances in this balance linked to progressive vision loss. Its activity interacts with proteins such as matrix metalloproteinases in these conditions contributing to the pathogenic processes.