Abcam, ab275039, Anti-C10 antibody [EPR23475-105] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal C10 antibody. Carrier free. Suitable for IP, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23475-105,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat,
Applications:Flow Cyt (Intra), IHC-P, IP, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab275039 is the carrier-free version of
ab275025
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C10 also known as complement component C10 plays an important role in the immune system by facilitating cell lysis through the membrane attack complex (MAC) assembly. It is part of the complement cascade traditionally involved in destroying pathogens and infected host cells. The protein has a molecular mass of approximately 30 kilodaltons. C10 expression occurs mainly in liver hepatocytes but its product circulates in the bloodstream and can also be found in various body fluids.
Biological function summary
This complement component carries out an essential function in innate immunity by contributing to the execution of cellular apoptosis and pathogen elimination. C10 becomes a part of the larger MAC complex interacting with other complement proteins such as C5b C6 C7 C8 and C9 to form pores on target cell membranes. These pore formations help in creating a passage for ions and molecules leading to osmotic lysis and subsequent cell death integral for immune defense.
Pathways
C10 integrates into the complement activation pathway particularly within the terminal pathway of the complement system. This pathway involves a cascade of protein interactions initiated by different triggers such as antigen-antibody complexes or pathogen surfaces. Related proteins like C3 and C5 facilitate the formation and activation of MAC focusing on pathogen neutralization. C10's mechanistic role ensures the effective completion of this terminal pathway contributing significantly to both classical and alternative complement pathways.
Abnormalities in C10 activity can relate to conditions such as paroxysmal nocturnal hemoglobinuria (PNH) and systemic lupus erythematosus (SLE). In PNH faulty complement regulation due to mutations in complement regulatory proteins like CD55 and CD59 leads to red blood cell destruction where C10's role in MAC formation aggravates hemolysis. In SLE autoantibody production and complement activation create immune complexes that accumulate in organs exacerbating inflammation and tissue damage. Understanding C10's interaction with these proteins provides insight into the therapeutic potential for managing such autoimmune-related conditions.