Abcam, ab275977, Anti-Integrin alpha 5 + beta 1 antibody [M200 (Volociximab)] - Rabbit IgG (Chimeric)

Size: 200µg
Rabbit Recombinant Monoclonal Integrin alpha 5 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Protein within Human ITGA5.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:M200 (Volociximab),
Isotype:IgG,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Protein within Human ITGA5. The exact immunogen used to generate this antibody is proprietary information.P08648,
Specificity:The antibody binds to human α5β1 integrin with a Kd of 0.367 nM (determined by Biacore), an EC50 of 0.2 nM and an IC50 of 2.3 nM.

Product details:
This rabbit monoclonal chimeric antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.02% Proclin 300Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Integrin alpha 5 beta 1 also known as alpha 5 beta 1 integrin is a cell surface receptor that facilitates cell adhesion and signaling. This integrin is a heterodimer composed of alpha 5 and beta 1 subunits each contributing to its structure and function. The molecular mass of integrin alpha 5 beta 1 is approximately 170 kDa. It predominantly expresses on the surface of fibroblasts endothelial cells and some epithelial cells where it plays an important role in mediating interactions between the cell and the extracellular matrix (ECM).
Biological function summary
Integrin alpha 5 beta 1 regulates processes like cell adhesion migration and differentiation. It binds to fibronectin in the ECM forming part of the adhesion complexes needed for cellular attachment and survival. This integrin couples with the actin cytoskeleton through talin and vinculin initiating signaling cascades that adjust cellular responses to changes in the microenvironment. Its mechanical linkage to the ECM helps sustain structural integrity affecting tissue homeostasis.
Pathways
Integrin alpha 5 beta 1 participates in the focal adhesion and the MAPK signaling pathway each with significant influence on cell behavior. In focal adhesion it partners with focal adhesion kinase (FAK) an important player in transmitting signals from the ECM to the intracellular environment. The MAPK pathway further involves integrin alpha 5 beta 1 integrating external signals to modulate gene expression and cell cycle progression. These pathways allow the integrin to coordinate cellular processes with the broader tissue context.
Integrin alpha 5 beta 1 associates with fibrotic diseases and cancer due to its role in cell adhesion and signaling. In fibrotic diseases such as pulmonary fibrosis altered expression and activity of integrin alpha 5 beta 1 can contribute to excessive ECM deposition and tissue stiffening. Similarly in cancer this integrin influences tumor progression and metastasis by modifying tumor microenvironment interactions. Proteins like fibronectin and transforming growth factor-beta (TGF-beta) intersect with integrin alpha 5 beta 1 activity impacting disease pathogenesis and progression.