Abcam, ab277077, Human Epiregulin ELISA Kit (EREG)

Size: 1 x 96Tests
Human Epiregulin ELISA Kit (EREG) is a single-wash 90-min Simplestep used to quantify Human Epiregulin (EREG) with a sensitivity of 5.803 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 5.803 pg/mL,
Range:6.25 - 400 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Epiregulin ELISA kit (ab277077) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Epiregulin protein in human serum and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Epiregulin with 5.8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human Epiregulin is a secreted peptide hormone encoded by the gene EREG. Epiregulin is a member of the epidermal growth factor family. Epiregulin is expressed as a type I membrane pro-form, proepiregulin, and epiregulin is catalytic cleaved to a 46- amino acid soluble form by disintegrin and metalloproteinase (ADAM). Epiregulin functions as a ligand of epidermal growth factor receptor (EGFR)and most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. Epiregulin contributes to inflammation, wound healing, tissue repair, and oocyte maturation.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Epiregulin also known as EREG is a member of the epidermal growth factor (EGF) family. It is a glycoprotein with a molecular weight of approximately 19 kDa. The protein is secreted and functions as a ligand for the EGF receptor (EGFR) and to a lesser extent for ErbB4 known as HER4 in humans. Epiregulin is expressed in a variety of tissues including placenta kidney and skin as well as within certain tumor cells. The expression can be regulated by signaling pathways involved in cellular growth and differentiation.
Biological function summary
Epiregulin participates in cellular processes such as proliferation differentiation and migration. It functions by binding to the EGFR initiating a cascade of downstream signaling events. As part of the EGF family it does not form part of a larger complex but it influences the activity of receptor dimers once bound. Its activity varies depending on the cellular context aiding in processes like tissue repair and embryonic development through its interaction with receptors and activation of signaling pathways.
Pathways
Epiregulin plays a significant role in the MAPK/ERK and PI3K/Akt signaling pathways both critical in regulating cell growth and survival. These pathways influence critical processes like cell cycle progression and apoptosis where epiregulin functions in coordination with other EGF family proteins such as TGF-alpha and amphiregulin. This protein assists in maintaining cellular homeostasis by modulating these pathways which are key to responding to environmental stimuli and cellular stress.
Epiregulin has associations with certain cancers such as colorectal and breast cancer. The protein often shows elevated expression levels in tumor samples implicating it in tumorigenesis. Its binding interaction with EGFR plays an important role in cancer cell survival and proliferation. Additionally epiregulin bears a connection to inflammatory diseases where its expression can exacerbate conditions like psoriasis through its impact on epidermal cells. In both cancer and inflammatory conditions the interplay between epiregulin and other related proteins like EGFR and ERBB2 influences disease progression and severity.