Abcam, ab277405, Human MEPE ELISA Kit

Size: 1 x 96Tests
Human MEPE ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human MEPE in Human in Cell Culture Media, Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 8.4 ng/mL,
Range:8.192 - 8000 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human MEPE ELISA Kit (ab277405) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human MEPE in serum, plasma (collect plasma using EDTA and Citrate as an anticoagulant; Heparin is not recommended for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Human MEPE coated on a 96-well plate. Standards and samples are pipetted into the wells and Human MEPE present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human MEPE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human MEPE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MEPE also known as Matrix Extracellular Phosphoglycoprotein is a protein involved in bone metabolism. It has a molecular mass of approximately 56 kDa. MEPE is expressed in osteoblasts and osteocytes cells critical for bone formation and maintenance. This protein also exists in other tissues like kidney and dental pulp where it could possibly have additional roles.
Biological function summary
MEPE contributes to the regulation of bone mineralization and phosphate homeostasis. The protein functions as part of a complex mechanism that modulates bone density responding to signals for bone growth or resorption. Its role involves binding to hydroxyapatite a mineral component of bone which suggests MEPE importantly influences bone matrix structure and strength.
Pathways
MEPE participates in the regulation of the phosphate and vitamin D pathways. It interacts closely with proteins such as PHEX (Phosphate-regulating gene with Homologies to Endopeptidases on the X chromosome) in these pathways. MEPE’s involvement in phosphate regulation affects both renal phosphate handling and intestinal absorption of dietary phosphate hinting at its role in broader systemic calcium and phosphate balance.
MEPE shows connections with disorders like X-linked hypophosphatemic rickets and tumor-induced osteomalacia. These conditions involve abnormal phosphate metabolism and bone degradation. The dysfunction of MEPE along with proteins like PHEX may result in impaired bone mineralization leading to clinical symptoms associated with these conditions such as bone pain and skeletal deformities.