Abcam, ab277408, Human Estrogen Receptor alpha ELISA Kit

Size: 1 x 96Tests
Human Estrogen Receptor alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Estrogen Receptor alpha in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 21 pg/mL,
Range:20.48 - 5000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Estrogen Receptor alpha ELISA Kit (ab277408) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human Estrogen Receptor alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Estrogen Receptor alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Estrogen Receptor alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Estrogen Receptor alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Estrogen Receptor alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Estrogen Receptor alpha also known as ERα or ESR1 is a nuclear receptor that acts as a transcription factor when activated by its ligand estrogen. Its molecular weight is approximately 66 kDa. ERα is expressed in various tissues such as breast tissue endometrium and ovarian cells as well as some areas of the central nervous system. The ERα protein binds to specific DNA sequences called estrogen response elements to regulate the transcription of target genes. Tools like ER alpha ELISA and assays using alpha peptides help to study this receptor.
Biological function summary
ERα plays a significant role in the regulation of estrogen signaling. Estrogens binding to ERα activate the receptor which can form a homodimer or heterodimer complex with other proteins like coactivators or corepressors. This complex then modulates the transcription of genes involved in cell growth proliferation and differentiation. ERα is closely linked with processes like reproductive tissue development and maintenance.
Pathways
ERα is involved in the estrogen signaling pathway and the cell cycle regulation pathway. In the estrogen signaling pathway ERα works together with proteins such as coactivators which enhance gene transcription and corepressors which can inhibit transcription. In the context of cell cycle regulation ERα's interactions with other cell cycle proteins help control cell division and proliferation linking ERα activity to the progression through different stages of the cell cycle.
ERα's dysregulation has been implicated in breast cancer and osteoporosis. ERα overexpression or mutations can lead to oncogenic effects in breast cancer making it a prominent therapeutic target. Drugs that modulate ERα activity like selective estrogen receptor modulators (SERMs) are used in breast cancer treatment. In osteoporosis ERα is related to bone density regulation with its activity affecting bone resorption and formation. Relationships between ERα and other proteins such as those involved in hormone signaling pathways impact these disease mechanisms.