Abcam, ab277416, Human MATN3/Matrilin-3 ELISA Kit

Size: 1 x 96Tests
Human MATN3/Matrilin-3 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human MATN3/Matrilin-3 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 20 pg/mL,
Range:20.48 - 5000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human MATN3/Matrilin-3 ELISA Kit (ab277416) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human MATN3/Matrilin-3 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human MATN3/Matrilin-3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human MATN3/Matrilin-3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human MATN3/Matrilin-3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human MATN3/Matrilin-3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Matrilin-3 also known as MATN3 is a disease-associated protein involved in cartilage extracellular matrix organization. Matrilin-3 is a member of the matrilin protein family which includes other family members like matrilin-1 and matrilin-4. The protein has a molecular mass of approximately 54 kDa. It is expressed mainly in cartilage tissue with significant presence in the growth plate cartilage which is important during development.
Biological function summary
Matrilin-3 contributes to the structural integrity of cartilage by forming filamentous networks in the extracellular matrix. It is part of a larger protein complex that includes collagen oligomeric matrix proteins and other structural components. Matrilin-3's affinity for interacting with other matrix components helps maintain cartilage homeostasis supporting overall tissue function and resilience.
Pathways
Matrilin-3 plays a role in organizing the extracellular matrix within the skeletal dysplasia pathway. This pathway includes other proteins like collagen types II and IX which work together to ensure proper cartilage framework and maintenance. Matrilin-3's involvement in these pathways highlights its role in skeletal development and the maintenance of cartilage health.
Matrilin-3 is linked to multiple epiphyseal dysplasia (MED) and osteoarthritis. Mutations in the MATN3 gene disrupt normal protein function and cartilage structure leading to MED a disorder characterized by joint pain and skeletal abnormalities. During osteoarthritis progression altered matrilin-3 expression correlates with cartilage degradation. Associated proteins like collagen type II are also implicated in these disease processes connecting matrilin-3 function with broader cartilage-related pathologies.