Abcam, ab277456, Human Peroxiredoxin 2/PRP ELISA Kit

Size: 1 x 96Tests
Human Peroxiredoxin 2/PRP ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Peroxiredoxin 2/PRP in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.34 ng/mL,
Range:0.328 - 80 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Peroxiredoxin 2/PRP ELISA Kit (ab277456) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human Peroxiredoxin 2/PRP in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Peroxiredoxin 2/PRP coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Peroxiredoxin 2/PRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Peroxiredoxin 2/PRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Peroxiredoxin 2/PRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Peroxiredoxin 2 (also known as PRP or PRDX2) is an antioxidant enzyme that plays an important role in reducing peroxides and protecting cells from oxidative damage. The target consisting of approximately 22kDa mass is expressed heavily in erythrocytes but can also be found in other tissues like the heart and liver. PRDX2 belongs to the peroxiredoxin family and its redox activity contributes significantly to cellular homeostasis and defense against oxidative stress.
Biological function summary
Peroxiredoxins like PRDX2 function by breaking down hydrogen peroxide and organic hydroperoxides safeguarding cells from oxidative harm. PRDX2 often forms homodimers or higher-order oligomers which influence its catalytic efficiency and chaperone activity. Within the cell it not only acts independently but also associates with other cellular components reflecting its participation in important cellular processes including cell proliferation and apoptosis.
Pathways
PRDX2 engages significantly within antioxidant defense systems specifically the thioredoxin pathway. It interacts with thioredoxin reductase and thioredoxin to facilitate the reduction of peroxides maintaining the cell’s redox balance. Furthermore PRDX2 intersects with cellular signaling pathways associated with inflammation and the regulation of cell death where proteins like NRF2 and KEAP1 play important roles in managing oxidant-inducible gene expression.
Disruptions in PRDX2 function connect to conditions like cancer and cardiovascular diseases. In cancer altered peroxiredoxin 2 expression correlates to tumor progression and resistance to chemotherapy implicating its interaction with proteins like NF-κB in enhancing cell survival. In cardiovascular diseases oxidative stress mediated by PRDX2 imbalance can lead to myocardial infarction and heart failure linking it to proteins such as SOD2 involved in mitochondrial defense.