Abcam, ab277477, Human OLFM2 ELISA Kit

Size: 1 x 96Tests
Human OLFM2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human OLFM2 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.45 ng/mL,
Range:0.41 - 100 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human OLFM2 ELISA Kit (ab277477) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human OLFM2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human OLFM2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human OLFM2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human OLFM2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human OLFM2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Olfactomedin 2 (OLFM2) also known as Noelin 2 is a protein encoded by the OLFM2 gene. It has a mass of approximately 60 kDa. OLFM2 localizes primarily to the extracellular matrix and the cytosol. Expression of this protein occurs in several tissues particularly in the nervous system and ocular tissues where it likely plays a role in the cellular makeup and maintenance.
Biological function summary
OLFM2 is involved in neural development and neuroprotection. It often functions as part of larger protein complexes facilitating cell-cell interactions and signal transduction processes. Additionally the protein influences axon guidance and synaptic organization contributing to the development and integrity of neural networks.
Pathways
OLFM2 integrates into various signaling pathways including the Wnt signaling pathway and the TGF-beta signaling pathway. These pathways regulate important processes like cell proliferation and differentiation. The protein often associates with other components like beta-catenin in the Wnt pathway reflecting its involvement in maintaining cellular homeostasis.
OLFM2 has links to conditions such as glaucoma and retinitis pigmentosa. In these cases OLFM2 interacts with proteins like myocilin which play roles in the pathology of these disorders. The disruption in OLFM2 function or expression can lead to altered cellular and molecular processes contributing to the disease's development and progression.