Abcam, ab277615, Recombinant human coronavirus SARS-CoV-2 PLpro protein (Active)

Size: 50µg
Recombinant human coronavirus SARS-CoV-2 PLpro protein (Active) is a SARS-CoV-2 Full Length protein, in the 1564 to 1882 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>80% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The protease specific activity of ab277615 was ≥450 pmol/min/mg at an enzyme concentration of 4 μg as per proteolytic activity assay protocol.,
Accession:P0DTD1,
Animal free:Yes,
Carrier free:No,
Species:SARS-CoV-2,
Storage buffer:pH: 7.5Preservative: 1.02% ImidazoleConstituents: 86.485% Water, 10% Glycerol (glycerin, glycerine), 1.75% Sodium chloride, 0.71% Sodium phosphate, 0.0015% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SARS-CoV-2 PLpro protein also known as papain-like protease plays an important role in the virus's ability to replicate and evade the host immune system. This protein is about 315 amino acids in length with a mass of approximately 35 kDa. You can find it expressed within infected host cells where it performs essential proteolytic processing of the viral polypeptide. Additionally PLpro modulates host cell pathways to assist the virus's ability to spread and persist within the host organism.
Biological function summary
The PLpro protein functions by cleaving the viral polyprotein at specific sites to generate non-structural proteins essential for replication. It forms part of a larger multi-functional complex within the viral replication machinery. This protease also counteracts host immune responses by deubiquitinating host proteins and modifying immune signaling molecules therefore suppressing antiviral signaling pathways. PLpro's activities not only support viral replication but also aid in the manipulation of host cell functions to benefit the virus.
Pathways
PLpro engages in viral replication and immune evasion pathways. It involves itself primarily in the viral replication transcription complex that SARS-CoV-2 depends on. By cleaving the viral polyprotein it works closely with the main protease Mpro another key protease essential for processing the viral polypeptides. Furthermore PLpro's deubiquitinating activity interferes with the host's innate immune signaling pathways which impacts the function of proteins like interferon regulatory factors (IRFs) that are important for mounting an effective antiviral response.
The SARS-CoV-2 PLpro protein plays a significant role in COVID-19 pathogenesis potentially altering the disease outcome by affecting viral replication and immune system interactions. Its action links to acute respiratory distress syndrome (ARDS) seen in severe COVID-19 cases where immune dysregulation occurs. Moreover through its immunomodulatory effects PLpro interacts with proteins involved in inflammatory pathways such as nuclear factor kappa B (NF-kB) suggesting its contribution to the inflammatory responses observed in COVID-19 patients.