Abcam, ab277862, Human LATS1 (WARTS) knockout THP-1 cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
LATS1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 31 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:THP-1,
Species or organism:Human,
Tissue:Blood,
Form:LiquidSee storage information,
Knockout validation:Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 31 bp deletion in exon 3,
Disease:Acute Monocytic Leukemia

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LATS1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LATS1/WARTS also known as Large Tumor Suppressor 1 is a serine/threonine protein kinase with a molecular mass of approximately 125 kDa. Its expression is found in various tissue types including skin lung and liver. Mechanically LATS1/WARTS plays a critical role in regulating the cell cycle and maintaining genomic stability. This kinase is an essential part of the Hippo signaling pathway and contributes to controlling the size of organs by inhibiting cell proliferation and promoting apoptosis.
Biological function summary
LATS1/WARTS functions as an important regulator of cellular growth and differentiation. It is part of the Hippo signaling complex which includes proteins like MST1/2 and YAP/TAZ. This complex helps maintain proper tissue homeostasis by modulating gene expression in response to cellular density and mechanical cues. In doing so LATS1/WARTS phosphorylates and inactivates downstream effectors YAP and TAZ preventing them from translocating to the nucleus where they could promote transcription of growth-promoting genes.
Pathways
Scientists have found LATS1/WARTS to be integral to both the Hippo signaling pathway and the cell cycle regulation pathway. Within these pathways LATS1/WARTS interacts closely with proteins such as MOB1 and YAP mediating cellular responses to growth inhibitory signals. By controlling these interactions LATS1/WARTS helps coordinate cell proliferation and apoptosis which is critical for proper developmental processes and prevention of tumor growth.
Dysregulation of LATS1/WARTS function is associated with the development of cancers particularly in the liver and breast. Research also links LATS1/WARTS to neurodegenerative conditions by implicating its role in apoptosis and cell survival. When its kinase activity is compromised the regulatory balance maintained by the Hippo pathway is disrupted potentially leading to unchecked cell proliferation. Connections with proteins such as YAP and TAZ further illustrate its involvement in these pathological conditions showing its importance in maintaining cellular integrity and function.