Abcam, ab277914, Human APC knockout HCT116 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
APC KO cell line available to order. KO validated by. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HCT116,
Species or organism:Human,
Tissue:Colon,
Form:LiquidSee storage information,
Antibiotic resistance:Puromycin 1µg/mL,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-APC, Gene editing type-Knockout, Gene editing method-CRISPR technology, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APC or Adenomatous Polyposis Coli is a multifunctional protein involved in several cellular processes. Its molecular weight is approximately 310 kDa. The APC protein is expressed in various tissues with higher concentrations found particularly in the colon and intestinal epithelial cells. Mechanically APC acts as a scaffold protein interacting with components of the cytoskeleton and regulating processes such as cell migration and adhesion. Its ability to bind to microtubules is key in its role in stabilizing them which affects cellular polarity and division.
Biological function summary
APC plays a significant role in the regulation of the Wnt signaling pathway a critical mediator of cell growth and differentiation. APC forms part of a larger destruction complex that includes proteins like Axin and GSK-3β. This complex degrades β-catenin preventing its accumulation in the cell nucleus and therefore modulating gene transcription involved in cell proliferation. The ability of APC to mediate this process is important for maintaining proper cellular homeostasis and preventing uncontrolled cell growth that may lead to neoplasms.
Pathways
APC is integral to the Wnt signaling and PI3K/Akt pathways. The Wnt pathway primarily concerns the regulation of cellular growth with APC facilitating the degradation of β-catenin therefore inhibiting the transcription of proliferative genes. Meanwhile in the PI3K/Akt pathway interactions between APC and β-catenin may influence cell survival and apoptosis. This connectivity to PI3K and Wnt pathways highlights APC's role in both promoting and inhibiting cell division processes depending on cellular context and signaling input.
APC is intrinsically linked to colorectal cancer and familial adenomatous polyposis (FAP). Mutations or loss of function in the APC gene lead to unregulated β-catenin levels promoting tumorigenesis in the colon. In colorectal cancer the absence or dysfunction of APC disrupts normal central pathways resulting in proliferative advantage to mutated cells. Connections to β-catenin further emphasize the mechanistic role APC plays in predisposing individuals to these diseases where abnormal Wnt pathway signaling becomes deleterious. Additionally disruption in APC function and the related pathways can alter interactions with other proteins like Axin and GSK-3β which are often involved in these disorders.