Abcam, ab278173, Inhibitory Immune Checkpoint panel 2 - Human WB

Size: 1Kit
Inhibitory Immune Checkpoint panel 2 - Human WB (ab278173) is part of the multiplex kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
Key facts
Reacts with:Human,
Target:CD47See target data

Product details:
Inhibitory Immune Checkpoint panel 2 - Human WB ab278173 contains multiple trial-sized versions of anti-human antibody clones against CD47, SIRP alpha, NKG2A, galectin 9/Gal-9, Indoleamine 2, 3-dioxygenase, 2B4 and CD276. For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.specifically selected for high performance in various applications. They are provided as a sampler panel to allow you to easily evaluate each antibody.
Panel contains:
- Rabbit monoclonal [EPR21794] to CD47 (20 μL)
ab218810
- Rabbit monoclonal [EPR16264] to SIRP alpha (20 μL)
ab191419
- Rabbit monoclonal [EPR23620-39] to NKG2A (20 μL)
ab252437
- Rabbit monoclonal [EPR22214] to galectin 9/Gal-9 (20 μL)
ab227046
- Rabbit monoclonal [EPR20374] to Indoleamine 2, 3-dioxygenase (20 μL)
ab211017
- Rabbit monoclonal [EPR23692-33] to 2B4 (20 μL)
ab256370
- Rabbit monoclonal [SP206] to CD276 (20 μL)
ab227670
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Use our intuitive search and select carrier-free or your label of choice. For bespoke conjugations or large volumes email bespoke@abcam.com.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD47 also known as integrin-associated protein (IAP) is a transmembrane protein with a mass of approximately 50 kDa. Located on the cell surface CD47 is expressed widely across various tissues. It interacts with signal regulatory protein alpha (SIRPα) which is predominantly expressed on myeloid and neural cells forming an immune checkpoint. Another significant protein NKG2A an inhibitory receptor plays a role in immune modulation. Indoleamine 23-dioxygenase (IDO) is an intracellular enzyme that catabolizes tryptophan. Meanwhile 2B4 an immune cell surface protein and Galectin-9 (Gal-9) modulate immune response. CD276 (B7-H3) acts as a co-stimulatory molecule and has a mass of roughly 80 kDa. These molecules are highly expressed in immune and tumor cells.
Biological function summary
CD47 modulates immune evasion by binding SIRPα sending inhibitory signals to macrophages to prevent phagocytosis. The NKG2A receptor with its ligand HLA-E contributes to immune tolerance by silencing NK and T cells. IDO by degrading tryptophan facilitates immune escape in the tumor microenvironment. The protein 2B4 allows natural killer (NK) and activated T cell signaling enhancing immune surveillance. Gal-9 acts as a potent immunosuppressive factor that negatively regulates T cell activity. CD276 fosters immune cell interactions often displaying anti-tumor properties in immune cells.
Pathways
CD47 and SIRPα interact within the phagocytosis inhibitory pathway important in regulating macrophage activity. NKG2A functions within the natural killer cell inhibitory pathway signaling through SHP-1 and SHP-2 phosphatases. IDO plays a vital role in the kynurenine pathway contributing to immune suppression. 2B4 functions in the signaling lymphocyte activation molecule (SLAM) family pathway impacting cell-mediated cytotoxicity. Gal-9 often cooperates with T cell immunoglobulin and mucin-domain containing-3 (TIM-3) to enhance immune suppression. CD276 as a member of the B7 family influences the co-stimulatory pathway regulating immune response.
CD47 associates with cancer tissues frequently contributing to tumor immune evasion. This interaction with SIRPα plays a role in preventing anti-tumor macrophage activity. NKG2A has been implicated in various cancers aiding immune escape by inhibiting NK cells. IDO contributes to immune tolerance in cancer by depleting tryptophan. 2B4 has links to autoimmune disorders where dysregulation affects immune balance. Gal-9 through its interaction with TIM-3 associates with autoimmune diseases by exacerbating immune tolerance. CD276 emerges as a potential therapeutic target in melanoma where it modulates immune responses to reduce tumor growth.