Abcam, ab278776, FITC Anti-Aurora B (phospho T232) + Aurora A (phospho T288) + IPL-1/STK13/Aurora C (phospho T198) antibody [AuroraABC-CC12]

Size: 10Test / 100Test
Rabbit Recombinant Monoclonal Aurora A phospho T288 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human AURKB phospho T232.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:AuroraABC-CC12,
Isotype:IgG,
Light chain type:kappa,
Conjugation:FITC,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:This product was produced with the following immunogens:The exact immunogen used to generate this antibody is proprietary information.Synthetic Peptide within Human AURKA phospho T288. Database linkO14965The exact immunogen used to generate this antibody is proprietary information.Synthetic Peptide within Human AURKC phospho T198. Database linkQ9UQB9

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 0.2% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aurora kinases are a family of serine/threonine kinases including Aurora A Aurora B and Aurora C which play key roles in mitotic regulation. Aurora A primarily functions in centrosome maturation and separation spindle assembly and ensuring accurate chromosomal segregation often located at spindle poles. Aurora B is integral to chromosome condensation spindle checkpoint and cytokinesis positioning itself at the centromere and later moving to the central spindle and midbody. Aurora C shares functions with Aurora B and is mainly found in testicular tissue. Aurora A and B have molecular masses around 46-48 kDa and Aurora C is slightly smaller. These kinases express in proliferative tissues with Aurora C found largely in the testes.
Biological function summary
Aurora kinases are necessary for accurate cell division. Aurora A contributes to centrosome duplication and mitotic entry forming a complex with other proteins like TPX2 to regulate microtubule dynamics. Aurora B a critical component of the chromosomal passenger complex includes partner proteins such as INCENP Survivin and Borealin. This complex controls the correct biorientation of chromosomes and execution of cytokinesis. Aurora C although less studied is known to function in meiotic cell division in germ cells often compensating for Aurora B's functions.
Pathways
Aurora kinases play important roles in the regulation of the cell cycle. Aurora A is part of the mitotic spindle checkpoint pathway and coordinates with other proteins such as PLK1 and CDC25B to ensure proper cell cycle progression. Aurora B is involved in the spindle assembly checkpoint interacting with proteins like BubR1 and Mad2 to monitor and correct kinetochore-microtubule attachments. These pathways ensure cells do not divide with errors preventing aneuploidy and maintaining genomic stability.
The dysregulation of Aurora kinases associates with cancer particularly due to their role in cell division. Aberrant expression or activation of Aurora A is linked to the development of several cancers including breast cancer connecting through proteins like p53 which can become dysfunctional. Aurora B overexpression also relates to malignancies such as colorectal cancer often working in conjunction with oncoproteins such as MYC. The correlation of these kinases with tumorigenesis makes them significant targets for cancer therapy research.