Abcam, ab279409, Mouse Versican ELISA Kit

Size: 1 x 96Tests
Mouse Versican ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Versican with a sensitivity of 12 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 12 pg/mL,
Range:125 - 8000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse Versican ELISA kit (ab279409) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Versican protein in mouse serum, plasma cell culture supernatatn samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Versican with 12 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Versican, also known as Versican Core Protein and Chondroitin Sulfate Proteoglycan Core Protein 2, is secreted protein that binds hyaluronic acid. It may play a role in intercellular signaling and in connecting cells with the extracellular matrix. Versican may take part in the regulation of cell motility, growth and differentiation.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C