Abcam, ab279857, Phospho-MARCKS (S152/156) and Total MARCKS ELISA Kit

Size: 1 x 96Tests
Phospho-MARCKS (S152/156) and Total MARCKS ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-MARCKS (S152/156) and Total MARCKS in Human in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Semi-quantitative,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Phospho-MARCKS (S152/156) and Total MARCKS ELISA Kit (ab279857) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated MARCKS protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an
in vitro
enzyme-linked immunosorbent assay for the measurement of phospho-MARCKS and total MARCKS. An anti-pan MARCKS antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and MARCKS present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho MARCKS (S152/156) antibody is added to detect phosphorylated MARCKS. In the remaining wells, biotinylated anti-pan-MARCKS antibody is used to detect pan MARCKS. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MARCKS (S152/156) or pan MARCKS bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C