Abcam, ab279886, Phospho-PDGFR beta (Y751) ELISA Kit

Size: 1 x 96Tests
Phospho-PDGFR beta (Y751) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-PDGFR beta (Y751) in Human in Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Human,
Assay type:Semi-quantitative,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Phospho-PDGFR beta (Y751) ELISA Kit (ab279886) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated PDGFR beta protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an
in vitro
enzyme-linked immunosorbent assay for the measurement of human phospho-PDGFR beta. An anti-pan PDGFR beta antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and PDGFR beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and rabbit anti-phospho-PDGFR beta (Y751) antibody is used to detect phosphorylated PDGFR beta. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGFR beta (Y751) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.
Biological function summary
The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.
Pathways
PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.
PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.