Abcam, ab280055, Mouse PRKAA1 knockout RAW 264.7 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PRKAA1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:RAW 264.7,
Species or organism:Mouse,
Tissue:Lymphatic,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PRKAA1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRKAA1 also known as the alpha 1 catalytic subunit of AMP-activated protein kinase (AMPK) plays an important role in cellular energy homeostasis. It has a molecular mass of approximately 63 kDa. The protein is widely expressed in various tissues including the liver skeletal muscle and brain. As a serine/threonine kinase PRKAA1 reacts to changes in cellular energy status by activating pathways that restore energy balance.
Biological function summary
PRKAA1 acts as an energy sensor and regulator in cells. As part of the AMPK heterotrimeric complex it regulates energy metabolism by phosphorylating various substrates involved in lipid glucose and protein metabolism. PRKAA1 activation results in increased glucose uptake fatty acid oxidation and mitochondrial biogenesis while inhibiting the synthesis of fatty acids cholesterol and triglycerides. This protein plays a significant role in maintaining cellular energy homeostasis and adapting to metabolic stress.
Pathways
PRKAA1 links to both the mTOR signaling pathway and the insulin signaling pathway. In the mTOR pathway it interacts with proteins such as TSC2 and Raptor leading to the inhibition of mTORC1 activity. In the insulin signaling pathway PRKAA1 affects the phosphorylation state of proteins involved in glucose homeostasis. PRKAA1's regulation of these pathways highlights its importance in metabolic processes and energy balance.
PRKAA1 shows association with metabolic disorders like type 2 diabetes and obesity. In type 2 diabetes dysregulation of PRKAA1-related pathways impacts insulin sensitivity and glucose uptake contributing to disease progression. Furthermore PRKAA1 relates to cardiac diseases often involving proteins such as PGC-1α which is important for mitochondrial biogenesis and energy metabolism in heart tissues. Understanding PRKAA1's role offers insights into potential therapeutic targets for these metabolic diseases.