Abcam, ab2807, Anti-alpha Adaptin antibody [AC1-M11]

Size: 500µL
Mouse Monoclonal alpha Adaptin antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 11 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow AP2A2.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:AC1-M11,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IHC-P, WB, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Cow AP2A2.Q0VCK5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha adaptin also known as AP2A1 is a component of the AP-2 complex carrying a molecular mass of about 110 kDa. This protein functions mechanically by mediating the formation of clathrin-coated vesicles at the plasma membrane. It facilitates the cargo selection process in clathrin-mediated endocytosis. Alpha adaptin is expressed broadly with significant presence in tissues like the brain and kidneys. This protein interacts strongly with clathrin and cargo receptors to regulate the internalization of various membrane-bound molecules.
Biological function summary
Alpha adaptin contributes to cellular homeostasis by managing the uptake and recycling of receptors and nutrients. It is an integral part of the adaptor protein complex 2 (AP-2) which orchestrates cargo binding and vesicle formation on the plasma membrane. This complex includes other subunits like beta adaptin mu adaptin and sigma adaptin all working collaboratively to ensure proper endocytic function. Alpha adaptin's role is essential in maintaining cell surface receptor levels and nutrient uptake.
Pathways
This protein is involved in endocytic pathways and is critical in synaptic vesicle recycling. Through this process it regulates neurotransmitter release by facilitating synaptic vesicle endocytosis a vital mechanism in neuron function. Alpha adaptin also interacts with dynamin another significant player in clathrin-mediated endocytosis which pinches off vesicles from the plasma membrane. This interaction supports its role in vesicle trafficking and cellular communication.
Alpha adaptin has implications in neurological disorders and cancer. Dysregulation of clathrin-mediated endocytosis involving alpha adaptin can influence the pathogenesis of neurodegenerative diseases like Alzheimer's disease. Perturbations in the AP-2 complex including alpha adaptin can misregulate receptor internalization impacting cell proliferation and migration therefore having potential links with oncogenesis. Moreover its interaction with proteins involved in receptor signaling pathways highlights its relevance to these conditions.