Abcam, ab280896, Anti-PHF8 antibody [EPR23913-57] - BSA and Azide free

Size: 100µg / 1mg
Knockout Tested Rabbit Recombinant Monoclonal PHF8 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Rat, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23913-57,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab280896 is the carrier-free version of
ab280887
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PHF8 also known as Plant Homeodomains Finger Protein 8 is a histone demethylase enzyme. It specifically removes methyl groups from histone H3 at lysine 9 (H3K9me2/me1) and at lysine 27 (H3K27me2/me1). PHF8 is a protein with a molecular mass of around 109 kDa. This protein is expressed widely in various tissues appearing notably in brain tissues and the testis which suggests a broad physiological role.
Biological function summary
PHF8 plays an important role in the regulation of gene expression through chromatin remodeling. It forms part of transcriptional complexes and directly interacts with transcription factors and chromatin proteins. Its activity modulates gene silencing and activation processes impacting multiple cellular functions such as cell cycle progression and neurodevelopment. The activities controlled by PHF8 make it critical for maintaining normal cellular function.
Pathways
PHF8 takes part in integral signaling and epigenetic regulation pathways. It is involved in the MAPK/ERK signaling pathway which affects cell proliferation and differentiation. PHF8 also connects with other proteins such as p53 influencing pathways that govern cell growth and apoptosis. Through these interactions PHF8 helps maintain proper balance in cell cycle and development processes.
Mutations or dysregulation of PHF8 have links to developmental disorders and certain cancers. Specifically PHF8 mutations have associations with X-linked mental retardation and cleft lip/palate conditions. Dysregulated PHF8 activity interacts with proteins like p53 to influence cancer progression particularly in prostate cancer. Understanding PHF8's role aids in the exploration of therapeutic targets for these conditions.